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nonnegative matrix factorization nnmf  (MathWorks Inc)


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    MathWorks Inc nonnegative matrix factorization nnmf
    Nonnegative Matrix Factorization Nnmf, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 96/100, based on 2034 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 96 stars, based on 2034 article reviews
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    Histology and immunohistochemical analysis of explanted scaffolds. (a) Representative micrographs of histological sections showing Hematoxylin/Eosin (H&E), human vimentin (hVim) and Pico Sirius Red (PSR) stains after 16 weeks subcutaneous implantation in mice. H&E and hVim images are shown at approximately identical locations on the samples. PSR images represent entire scaffolds by stitched together micrographs. (b) , Quantitative analysis of collagen content from Raman spectroscopy (NNMF Component 3) and PSR images after 16 weeks of implantation. Collagen (Coll.) content from PSR histology is represented by a ratio of total PSR positive area divided by total scaffold cavity perimeter (Area-to-perimeter ratio). Histology derived measures of collagen content correlate well with both ex vivo and in vivo Raman derived collagen estimates. Pearson's correlation coefficient (r). Scales bars: 200 µm (H&E, hVim micrographs) and 1 mm (PSR micrographs). Groups: scaffolds without (No cells ) human mesenchymal stem cells (hMSCs), with hMSCs (MSC 1.5 , MSC 7.5 , MSC 7.5 +GF ) amount indicated by subscript ( e.g. 7.5 = 7.5 × 10 5 cells per scaffold). hMSCs preconditioned with BMP2 growth factor for 24 h prior to implantation (+GF).

    Journal: Biomaterials and Biosystems

    Article Title: In vivo non-invasive monitoring of tissue development in 3D printed subcutaneous bone scaffolds using fibre-optic Raman spectroscopy

    doi: 10.1016/j.bbiosy.2022.100059

    Figure Lengend Snippet: Histology and immunohistochemical analysis of explanted scaffolds. (a) Representative micrographs of histological sections showing Hematoxylin/Eosin (H&E), human vimentin (hVim) and Pico Sirius Red (PSR) stains after 16 weeks subcutaneous implantation in mice. H&E and hVim images are shown at approximately identical locations on the samples. PSR images represent entire scaffolds by stitched together micrographs. (b) , Quantitative analysis of collagen content from Raman spectroscopy (NNMF Component 3) and PSR images after 16 weeks of implantation. Collagen (Coll.) content from PSR histology is represented by a ratio of total PSR positive area divided by total scaffold cavity perimeter (Area-to-perimeter ratio). Histology derived measures of collagen content correlate well with both ex vivo and in vivo Raman derived collagen estimates. Pearson's correlation coefficient (r). Scales bars: 200 µm (H&E, hVim micrographs) and 1 mm (PSR micrographs). Groups: scaffolds without (No cells ) human mesenchymal stem cells (hMSCs), with hMSCs (MSC 1.5 , MSC 7.5 , MSC 7.5 +GF ) amount indicated by subscript ( e.g. 7.5 = 7.5 × 10 5 cells per scaffold). hMSCs preconditioned with BMP2 growth factor for 24 h prior to implantation (+GF).

    Article Snippet: Following pre-processing, spectral models were developed using the MATLAB statistics toolbox function non-negative matrix factorization (NNMF) ( c) with in vivo spectra ( a), ex vivo , and reference spectra ( b) as input.

    Techniques: Immunohistochemical staining, Raman Spectroscopy, Derivative Assay, Ex Vivo, In Vivo